推荐产品
生物源
bovine pancreas (trypsin)
品質等級
形狀
buffered aqueous suspension
濃度
≥15 units/mL (packed gel)
標籤範圍
≥15 units per mL (packed gel)
基質
beaded agarose
儲存溫度
2-8°C
一般說明
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
應用
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
單位定義
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
外觀
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
準備報告
TPCK-treated
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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