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一般說明
3,3′,5,5′-四甲基联苯胺(TMB)是辣根过氧化物酶(HRP)偶联物的显色底物。它会产生永久性不可溶解的深蓝色反应产物，可应用于比色定量。
應用
3,3′,5,5′-四甲基联苯胺(TMB)显色液（转印膜用底物）可作为蛋白质免疫印迹分析(western blot)的显色底物。也可用作酶联免疫吸附测定(ELISA)的底物溶液。
3,3′,5,5′-四甲基联苯胺(TMB)细胞膜的液体底物体系：
- 用于酶联免疫斑点法(ELISPOT)分析
- 用作IgG过氧化物酶的底物
- 应用于免疫复合物的可视化
外觀
即用型。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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