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Merck

SRP5172

Sigma-Aldrich

Catenin β, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

别名:

CTNNB, FLJ25606

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected Sf9 cells

化驗

≥70% (SDS-PAGE)

形狀

buffered aqueous glycerol solution

分子量

~115 kDa

技術

activity assay: suitable

溶解度

water: soluble

適合性

suitable for molecular biology

NCBI登錄號

應用

cell analysis

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... CTNNB1(1499)

一般說明

Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions. CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. The gene encoding β-catenin is localized on human chromosome 3p22.1.

應用

Catenin β, GST tagged human has been used:
  • as a component of protein kinase buffer for in vitro kinase assay
  • in in vitro citrullination assay to characterize β-catenin citrullination
  • to perform differentiation factor screening

生化/生理作用

β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome. APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas. The protein has a role in homeostasis and development.

外觀

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

準備報告

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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访问文档库

Yi Qu et al.
Nature chemical biology, 14(1), 94-101 (2017-10-31)
Wnt (wingless)/β-catenin signaling is critical for tumor progression and is frequently activated in colorectal cancer as a result of the mutation of adenomatous polyposis coli (APC); however, therapeutic agents targeting this pathway for clinical use are lacking. Here we report
Mutations in beta-catenin are uncommon in colorectal cancer occurring in occasional replication error-positive tumors.
Kitaeva MN
Cancer Research (1997)
Somatic mutations of the ?-catenin gene are frequent in mouse and human hepatocellular carcinomas
Alix de La Coste
Proceedings of the National Academy of Sciences of the USA (1998)
Connective Tissue Growth Factor Promotes Efficient Generation of Human Induced Pluripotent Stem Cell-Derived Choroidal Endothelium
Allison E Songstad
Stem Cells Translational Medicine (2017)
Regulation of ?-Catenin Function by the I?B Kinases*
Carmela Lamberti
The Journal of Biological Chemistry (2001)

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