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生物源
human
重組細胞
expressed in baculovirus infected insect cells
化驗
≥60% (SDS-PAGE)
形狀
aqueous solution
比活性
≥1770 pmol/min-μg
分子量
129 kDa (p110α)
88 kDa (p85α)
包裝
pkg of 20 μg
濃度
0.38 mg/mL
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... PIK3CA(5290)
一般說明
Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.
應用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
單位定義
One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
外觀
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
準備報告
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
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