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Key Documents

SRP0235

Sigma-Aldrich

PI3 kinase [p110a(H1047R)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

别名:

PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥60% (SDS-PAGE)

形狀

aqueous solution

比活性

≥1770 pmol/min-μg

分子量

129 kDa (p110α)
88 kDa (p85α)

包裝

pkg of 20 μg

濃度

0.38 mg/mL

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PIK3CA(5290)

一般說明

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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