SRE0113

Bst Max DNA Polymerase

recombinant, expressed in E. coli, Salt and Inhibitor Tolerant

• 别名: Bst DNA polymerase, Isothermal DNA Polymerase
• 分類程式碼代碼: 12352200
• NACRES: NA.54

属性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 比活性: 40,000 U/mg
• 特點: Salt and Inhibitor Tolerant
• 最適pH: 8.5
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Loop-mediated isothermal amplification (LAMP) has emerged as a key alternative to polymerase chain reaction (PCR)-based techniques in the amplification of nucleic acids. While conventional PCR employs a thermal cycler for repetitive cycling temperatures when amplifying, isothermal amplification techniques such as LAMP occur at a single and fixed temperature allowing the use of simple, portable, and more robust instruments for fast and exponential amplification.
The Bst Max DNA polymerase is an in-silicon designed homolog of Bst DNA Polymerase (large fragment) suitable for DNA amplification at elevated temperatures with an optimum of 65°C. Bst Max DNA polymerase is a salt-tolerant recombinant polymerase (optimal salt concentration 50-350 mM) with more than two-fold enhanced strand-displacement activity and processivity when compared to Bst polymerase. The Bst enzyme is active from 25 to 65°C. It lacks 5’-3’- and 3’-5’-exonuclease activity.

應用

Bst Max DNA Polymerase is

• Ideal for isothermal applications such as LAMP and RT-LAMP for its superior amplification performance and robustness, especially at point-of-care diagnostics
• Compatible with a wide range of buffer formulations and isothermal applications such as Ramification amplification (RAM), NEMA (Nicking enyzme-mediated amplification), HAD (Helicase-dependent amplification), MDA/SDA (Multiple/Strand displacement amplification), RCA (Rolling circle amplification), RPA (Recombinase Polymerase amplification)

特點和優勢

• Efficient strand displacement activity allows the polymerase to amplify at a faster rate while displacing the second DNA strand during synthesis.
• High processivity ensures that the polymerase can amplify more nucleotides consecutively without dissociation from the DNA template
• Higher salt and inhibitor tolerance ensures enhanced amplification performance without the need for sample prep steps
• Ideal for amplification of most sample types, especially small and impure samples
• Triton-free formulation complies with the European Chemicals Agency’s REACH regulations to improve the protection of human health and the environment from risks that can be posed by chemicals

單位定義

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

重構

In solution.

其他說明

Order primers for LAMP using our online configurator. For assistance with primer design prior to purchase, please use the online design tool from our partner.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable