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SHPM15

Sigma-Aldrich

MISSION® LentiPlex® Mouse Pooled shRNA Library TRC1.5

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

分類程式碼代碼:
12352200

品質等級

200

產品線

MISSION®

濃度

≥5x108 VP/ml (via p24 assay)

技術

capture ELISA: 5 x 108 TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

相关类别

一般說明

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Mouse shRNA Pooled Library 2.0 includes additional gene and clone coverage from the previously offered LentiPlex 1.0 Pooled Library . With the addition of 2.0, which consists of 7,000+ mouse genes and 25,000+ total clone coverage divided into 3 subpools, the complete whole-genome mouse library complete whole-genome mouse librarynow consists of over 110,000 shRNA constructs from the TRC collection targeting 21,000+ mouse genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The complete library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into fifteen subpools of approximately 5,500-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.

其他說明

The MISSION LentiPlex Mouse Pooled shRNA Libraries consist of Catalog Numbers SHPM01, SHPM15, SHPM2, and SHPMLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library.

Amplification and sequencing primers are also provided for downstream hit identification.

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

推薦產品

Browse additional MISSION shRNA Gene Family Sets in our MISSION shRNA portal

法律資訊

Lentiviral License Agreement
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells.
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology

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