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Merck

SHC216V

Sigma-Aldrich

MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Transduction Particles

Targets no known genes from any species

别名:

MISSION®

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About This Item

分類程式碼代碼:
41106609
NACRES:
NA.51

品質等級

產品線

MISSION®

濃度

≥1x106 VP/ml (via p24 assay)

運輸包裝

dry ice

儲存溫度

−70°C

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一般說明

The MISSION® TRC2 pLKO.5-puro Non-Target shRNA Control Tranduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 uL at 1 x 106 TU/mL via p24 assay.
当使用MISSION® TRC shRNA克隆进行实验时,选择适当对照品是您的实验设计的关键要素,以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱,请访问 sigma.com/shrna

應用

To see more application data, protocols, vector maps visit sigma.com/shrna.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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