SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• 别名: Alanine Racemase from Bacillus stearothermophilus
• CAS号: 9024-06-0
• EC 号: 5.1.1.1 (BRENDA, IUBMB)
• MDL號碼: MFCD03454300
• 分類程式碼代碼: 12352204
• NACRES: NA.54

属性

• 生物源: Bacillus sp. (B. stearothermophilus)
• 品質等級: 200
• 形狀: buffered aqueous solution
• 比活性: ≥10 IU/mg solid
• 分子量: M_r 78 kDa (2 subunits 39 kDa each)
• 儲存溫度: −20°C

说明

應用

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

生化／生理作用

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

單位定義

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

外觀

Aqueous solution containing 10 mM potassium phosphate

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable