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生物源
mouse
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
PD-65, monoclonal
形狀
buffered aqueous solution
分子量
~42 kDa
物種活性
bovine, rat, human, mouse
包裝
antibody small pack of 25 μL
濃度
1 mg/mL
技術
immunoblotting: 0.25-0.5 μg/mL using using human HEK-293T cells.
immunofluorescence: 1-2 μg/mL using human HEK-293T cells.
同型
IgG2a
UniProt登錄號
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... NPHS2(7827)
一般說明
足细胞是覆盖肾脏肾小球外部的高度专用化内脏上皮细胞。相邻足细胞的足突会经常发生相互交叉，留下了由狭缝薄膜桥连的滤过隙。这是一种细胞外粘附样连接，可作为大分子的屏障并在肾小球滤过过程中起关键作用。Podocin（NPHS2蛋白）是一组与狭缝膜有关的蛋白质中的重要成员。Podocin属于脂筏相关蛋白的band-7-stomatin家族。它是一种带有细胞内N和C末端的发卡样整合膜蛋白。Podocin位于狭缝膜的插入位点，被认为是维持或调节狭缝膜结构完整性所需的一种支架蛋白。它在那里会与肾病蛋白（NPHS1蛋白）相互作用，而肾病蛋白对肾脏滤过屏障和衔接蛋白CD2-AP的发育和功能至关重要。Podocin被锚定在质膜外小叶上富含糖鞘脂和胆固醇的脂筏中，并可用肌动蛋白细胞骨架进行部分定位。体外研究表明它参与了HEK细胞中通过AP-1的肾病蛋白信号转导促进。除了存在于肾脏中外，它还被报道存在于大鼠神经组织以及可能的血管平滑肌中。
免疫原
与KLH偶联的人Podocin的合成肽
應用
该抗体可用于各种免疫化学技术，包括免疫印迹（~42kDa）和免疫荧光。通过免疫印迹对Podocin条带的检测可被免疫原特异性的抑制。
外觀
本品为溶液形式，溶于0.01M磷酸盐缓冲盐水，pH 7.4，含有15 mM叠氮化钠作为防腐剂
其他說明
本产品仅供研发使用，不得用于药物、家庭或其他用途。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Hiroshi Kawachi et al.
Journal of the American Society of Nephrology : JASN, 14(1), 46-56 (2002-12-31)
Podocin is identified as a product of the gene mutated in a patient with autosomal recessive steroid-resistant nephrotic syndrome. Although podocin is reported to be located at the slit diaphragm area, the precise role of podocin for maintaining the barrier
Séverine Roselli et al.
Traffic (Copenhagen, Denmark), 5(1), 37-44 (2003-12-17)
Podocytes are specialized epithelial cells of the glomerulus in the kidney, which interconnect at the top of the glomerular basement membrane through the slit diaphragm, an adherens-like junction that plays a crucial role in the glomerular filtration process. Podocin, a
Izumi Horinouchi et al.
Kidney international, 64(6), 2092-2099 (2003-11-25)
Mutations of the NPHS2 gene are responsible for autosomal-recessive steroid-resistant nephrotic syndrome. Its product, podocin, faces the slit diaphragm area with its two ends in the cytoplasm of foot processes. We generated rabbit polyclonal antibodies against conjugated peptides from human
G Caridi et al.
Journal of the American Society of Nephrology : JASN, 12(12), 2742-2746 (2001-12-01)
Podocin mutations (NPHS2 gene) are responsible for the autosomal recessive form of steroid-resistant nephrotic syndrome. As a result of a screening for these gene alterations in a cohort of Italian patients with nonfamilial nephrotic syndrome and histologic focal segmental glomerulosclerosis
Séverine Roselli et al.
Molecular and cellular biology, 24(2), 550-560 (2004-01-01)
Podocytes are specialized epithelial cells covering the basement membrane of the glomerulus in the kidney. The molecular mechanisms underlying the role of podocytes in glomerular filtration are still largely unknown. We generated podocin-deficient (Nphs2-/-) mice to investigate the function of
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