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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 90 kDa
物種活性
rat, mouse, human
加強驗證
independent
Learn more about Antibody Enhanced Validation
濃度
~1 mg/mL
技術
immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.
indirect immunofluorescence: 5-10 μg/mL using human HeLa cells.
western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... STIM1(6786)
mouse ... Stim1(20866)
rat ... Stim1(117086)
一般說明
STIM1 (stromal interaction molecule 1) is a conserved single-pass transmembrane protein, which is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 is modified by phosphorylation and N-linked glycosylation. STIM1 localized predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels.
免疫原
synthetic peptide corresponding to amino acids 61-74 of human STIM1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
應用
Anti-STIM1 (N-terminal) antibody produced in rabbit has been used in:
- western blotting
- immunoprecipitation
- immunofluorescence
生化／生理作用
STIM1 (stromal interaction molecule 1) is required for the activation of store-operated Ca2+ influx. Overexpression of STIM1 and Orai1 markedly increases the calcium release-activated calcium (CRAC) current (I-CRAC).
外觀
Solution in 0.01 M phosphate buffered saline, 7.4 pH, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
其他客户在看
Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1
Mercer JC, et al.
The Journal of Biological Chemistry, 281(34), 24979-24990 (2006)
Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins
Williams RT, et al.
The Biochemical Journal, 357(3), 673-685 (2001)
Vivek Krishnan et al.
eLife, 11 (2022-02-12)
Peripheral coupling between the sarcoplasmic reticulum (SR) and plasma membrane (PM) forms signaling complexes that regulate the membrane potential and contractility of vascular smooth muscle cells (VSMCs). The mechanisms responsible for these membrane interactions are poorly understood. In many cells
Inhibition of store-operated calcium entry attenuates MPP+-induced oxidative stress via preservation of mitochondrial function in PC12 cells: involvement of Homer1a
Li X, et al.
PLoS ONE, 8(12), e83638-e83638 (2013)
Alicia Sampieri et al.
Scientific reports, 8(1), 13252-13252 (2018-09-07)
The involvement of inositol trisphosphate receptor (IP3R) in modulating store-operated calcium entry (SOCE) was established many years ago. Nevertheless, the molecular mechanism responsible for this observation has not been elucidated to this date. In the present study we show that
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