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用途
sufficient for 100 reactions
sufficient for 20 reactions
sufficient for 500 reactions
特點
dNTPs included
hotstart
技術
DNA amplification: suitable
PCR: suitable
RT-PCR: suitable
RT-qPCR: suitable
cDNA synthesis: suitable
qPCR: suitable
輸入
purified RNA
相容性
ABI 7000
ABI 7300
ABI 7500
ABI 7700
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad iCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
QuantStudio™
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.
應用
ABScript II One Step RT-qPCR Probe Kit is designed for use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed.
For Research Use Only.
Application Note
Additional Materials Required but Not Included:
Compatible Instruments:
*50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne™/StepOnePlus™
**50X ROX Reference Dye II
Applied Biosystems 7500/ViiA™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene® 3000
NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96™/ CFX384™, Roche® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers®.
For Research Use Only.
Application Note
Additional Materials Required but Not Included:
- RNA templates, primers and probes
- Optical-grade qPCR tubes, plates, sealing films, and aerosol-resistant pipette tips
Compatible Instruments:
*50X ROX Reference Dye I
Applied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne™/StepOnePlus™
**50X ROX Reference Dye II
Applied Biosystems 7500/ViiA™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene® 3000
NO ROX Reference Dye
Bio-Rad iCyclers/ CFX96™/ CFX384™, Roche® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers®.
特點和優勢
- Ready-to-use one-step RT-qPCR probe kit with all components for RT-qPCR (except primers, probes and template RNA)
- Uses the robust ABScript II Reverse Transcriptase (RT), which provides reliable and efficient reverse transcription on a wide range of RNA template amount
- This one-step kit improves sensitivity and effectively prevents contamination
- Flexible format, as the ABScript II One Step RT-qPCR Probe Kit includes two separate 50X concentrations of the ROX (passive reference) dye, to be added as required by specific qPCR instruments.
成分
2X One Step RT-qPCR Probe Buffer*
One Step Probe HS Taq
ABScript II RT Enzyme Mix
50X ROX Dye I *
50X ROX Dye II **
RNase-free ddH2O
See datasheet for more information.
Reaction Size
Reaction size is based on a 50 uL reaction volume.
The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:
20RXN: 0.5 mL
100RXN: 2.5 mL
500RXN: 1.25 mL
One Step Probe HS Taq
ABScript II RT Enzyme Mix
50X ROX Dye I *
50X ROX Dye II **
RNase-free ddH2O
See datasheet for more information.
Reaction Size
Reaction size is based on a 50 uL reaction volume.
The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:
20RXN: 0.5 mL
100RXN: 2.5 mL
500RXN: 1.25 mL
法律資訊
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
Mastercycler is a registered trademark of Eppendorf AG
QuantStudio is a trademark of IROA Technologies LLC
Roche is a registered trademark of Roche Molecular Systems, Inc.
Rotor-Gene is a registered trademark of Qiagen GmbH
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ViiA is a trademark of ThermoFisher
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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