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物種活性
human
包裝
kit of 96 wells (12 strips x 8 wells)
技術
ELISA: suitable
capture ELISA: suitable
輸入
sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... RPS27A(6233)
一般說明
The Human Ubiquitin+1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Ubiquitin+1 in serum, plasma, cell culture supernatants and urine.
The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.
The RPS27A (ribosomal protein S27a) gene is mapped to human chromosome 2p16.1. The protein complex encoded contains ubiquitin at the N-terminus and a C-terminal ribosomal protein S27a.
免疫原
Recombinant Human Ubiquitin
應用
请参考Protocol了解详情。
生化／生理作用
Ribosomal protein S27a (RPS27a), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression and inhibits apoptosis of leukemia cells. Overexpression of RPS27A results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of UBCEP-1, which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.
The ribosomal protein S27a (Rps27a) possesses two main functions, it is responsible for ribosome formation and takes part in post translational modification of proteins. DNA damage elevates the RPS27A gene expression. The RPS27A gene is upregulated in chronic myelogenous leukemia and colorectal cancer. It is known to stimulate cell cycle by inducing cellular checkpoints through p53 and cell proliferation and prevent apoptosis in leukemia cells.
其他說明
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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产品编号
说明
化学品安全说明书
訊號詞
Warning
危險聲明
防範說明
危險分類
Met. Corr. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
Overexpression of RPS27a contributes to enhanced chemoresistance of CML cells to imatinib by the transactivated STAT3.
Wang H, et al.
Oncotarget, 7(14), 18638-18638 (2016)
DNA damage stress induces the expression of ribosomal protein S27a gene in a p53-dependent manner.
Nosrati N, et al.
Gene, 559(1), 44-51 (2015)
A novel component of the ubiquitin pathway, ubiquitin carboxyl extension protein 1 is overexpressed in prostate cancer.
Ko Y, et al.
International Journal of Molecular Medicine, 15(2), 183-196 (2005)
Identification and expression of MMSA-8, and its clinical significance in multiple myeloma.
He R, et al.
Oncology Reports, 37(6), 3235-3243 (2017)
RPS27a promotes proliferation, regulates cell cycle progression and inhibits apoptosis of leukemia cells.
Wang H, et al.
Biochemical and Biophysical Research Communications, 446(4), 1204-1210 (2014)
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