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用途
sufficient for 10 purifications
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
蛋白A抗体纯化试剂盒为分离单克隆和多克隆抗体提供所有所需试剂。
特異性
容量/柱（按物种分类）
人IgG 20-25 mg
小鼠IgG1 8-12 mg
小鼠IgG2a 10-15 mg
小鼠IgG2b 6-10 mg
小鼠IgG3 15-20 mg
兔IgG 8-12 mg
山羊IgG 2-4 mg
牛IgG 8-12 mg
人IgG 20-25 mg
小鼠IgG1 8-12 mg
小鼠IgG2a 10-15 mg
小鼠IgG2b 6-10 mg
小鼠IgG3 15-20 mg
兔IgG 8-12 mg
山羊IgG 2-4 mg
牛IgG 8-12 mg
應用
PURE-1A以预先包装、易于使用的试剂盒形式提供蛋白A技术。使用该试剂盒，可以从血清、腹水或细胞培养物上清液中纯化出毫克量的IgG。
可能可以从其他物种纯化IgG，但是，研究人员必须确定该试剂盒对其应用的适用性。
蛋白A抗体纯化试剂盒适用于纯化人、小鼠、兔、山羊和牛的IgG。
特點和優勢
- 高容量 - 每柱纯化多达8 mg小鼠IgG或25 mg人IgG
- 特异性 - 仅结合IgG
- 易于使用 - 仅需一步即可完成抗体洗脱和脱盐，随时可用
- 温和 - 避免长时间暴露于低pH值的抗体
原則
使用我们的柱式系统，可以在生理pH下将抗体作为高度纯化的蛋白质洗脱。蛋白A是从哺乳动物宿主中分离抗体的有力工具。蛋白A对IgG具有高度特异性，并确保抗体制剂几乎不含IgA、IgM和非免疫球蛋白血清蛋白，如白蛋白。
免責聲明
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
仅试剂盒组分
产品编号
说明
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
查看所有结果 (8)
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 4 Oral - STOT SE 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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实验方案
Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.
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