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GE28-9852-54

rProtein A GraviTrap

Cytiva 28-9852-54, pack of 10 × 1 mL

别名:

GraviTrap

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

形狀

prepacked column

交聯

cross-linked

包裝

pack of 10 × 1 mL

製造商/商標名

Cytiva 28-9852-54

儲存條件

(20% Ethanol)

技術

liquid chromatography (LC): suitable

床體積

1 mL

基質

(Highly cross-linked agarose, 4%)

基質活性組

Protein A functional group

粒徑

(90 µm)

容量

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

分離技術

affinity

儲存溫度

2-8°C

一般說明

rProtein A GraviTrap includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose Fast Flow.

應用

rProtein A GraviTrap are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

特點和優勢

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap ~ 50 mg human IgG/column

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

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