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Merck

P8047

Sigma-Aldrich

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−R-Phycoerythrin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

别名:

Goat Anti-Human IgG

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

phycoerythrin (R-PE) conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

技術

direct immunofluorescence: 1:32

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

IgG is a glycoprotein antibody that contains two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4.

免疫原

Purified human IgG

應用

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment R-Phycoerythrin antibody produced in goat has been used in bead-based assay and immunoassay multiplex magpix
Anti-Human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody is suitable for use in flow cytometry . The antibody can also be used for direct immunofluorescence (1:32).

生化/生理作用

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallisable (fc), comprises the H chain constant region.
IgG antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . The use of anti-human IgG (γ-chain specific), F(ab′)2 fragment-R-Phycoerythrin antibody helps avoid background staining due to the presence of Fc receptors. The product is specific for human IgG when tested against purified human IgA, IgG, IgM, Bence Jones κ, and Bence Jones λ myeloma proteins.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.1 mM EDTA, 1 mM iodoacetamide, 1% bovine serum albumin and 15 mM sodium azide

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Bartholomew N Ondigo et al.
PeerJ, 7, e6120-e6120 (2019-01-11)
New reagents have emerged allowing researchers to assess a growing number of vaccine-associated immune parameters. Multiplex immunoassay(s) are emerging as efficient high-throughput assays in malaria serology. Currently, commercial vendors market several bead reagents for cytometric bead assays (CBA) but relative
Anne E P Frosch et al.
The American journal of clinical nutrition, 100(3), 968-973 (2014-08-01)
Achieving optimal iron status in children in malaria-endemic areas may increase the risk of malaria. Malaria itself may contribute to iron deficiency, but the impact of an interruption in malaria transmission on the prevalence of iron deficiency is unknown. We
Bartholomew N Ondigo et al.
The Journal of infectious diseases, 210(7), 1123-1132 (2014-04-17)
Tools that estimate recent and long-term malaria transmission in a population would be highly useful for malaria elimination programs. The prevalence of antibodies to 11 Plasmodium falciparum antigens was assessed by cytometric bead assay or enzyme-linked immunosorbent assay in 1000
William Spooner et al.
Nature communications, 9(1), 4128-4128 (2018-10-10)
Selecting the most appropriate protein sequences is critical for precision drug design. Here we describe Haplosaurus, a bioinformatic tool for computation of protein haplotypes. Haplosaurus computes protein haplotypes from pre-existing chromosomally-phased genomic variation data. Integration into the Ensembl resource provides
Optimization of a magnetic bead-based assay MAGPIX textregistered-Luminex) for immune surveillance of exposure to malaria using multiple Plasmodium antigens and sera from different endemic settings
Varela ML, et al.
Malaria Journal, 17(1), 324-324 (2018)

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