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P6407

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

别名:

PDL

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About This Item

线性分子式:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS号:
27964-99-4
MDL號碼:
MFCD00131934
分類程式碼代碼:
12352202
PubChem物質ID:
24898764
NACRES:
NA.75

product name

聚-D-赖氨酸 氢溴酸盐, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

生物源

synthetic (organic)

品質等級

200

無菌

γ-irradiated

產品線

BioReagent
BioXtra

形狀

lyophilized powder

分子量

70,000-150,000

包裝

pkg of 10 X 5 mg
pkg of 5 mg

濃度

0.016—0.032 mmol lysine

技術

cell culture | mammalian: suitable

表面覆盖率

4 μg/cm2

雜質

<10% water (by Karl Fischer)

溶解度

H2O: soluble 50 mg/mL, clear, colorless

運輸包裝

ambient

儲存溫度

−20°C

SMILES 字串

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI 密鑰

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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應用

多聚-D-赖氨酸氢溴酸盐已用于:

  • 作为培养板的涂层用于培养大鼠神经胶质前体细胞(RGPCS)。
  • 用于包被继代培养伸长细胞的培养瓶和培养板
  • 处理盖玻片的表面以便细胞附着
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。

建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。

生化／生理作用

多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。

特點和優勢

  • 用伽马（γ）射线消毒
  • 在2-8˚C下无菌多聚-D-赖氨酸溶液可保存三年。

成分

聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

注意

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

準備報告

该产品具有70,000-150,000的分子量并经细胞培养检测和γ射线照射。如需去除HBr，将该产品溶解于中性缓冲液并通过透析以去除这些盐。 通常，如需将该产品作为附着因子使用，可将50 mL无菌组织培养级用水加入5 mg的聚赖氨酸中，并用1 mL溶液对每25 cm2的表面进行无菌涂覆。 在5分钟后，通过吸液将溶液去除并彻底冲洗表面。 在加入细胞和培养基前让其干燥两小时。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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