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形狀
lyophilized powder
比活性
≥60 units/mg protein
成份
Protein, 20-40% biuret
異物活動
ATPase ≤0.5%
phosphorylase a ≤1%
phosphorylase b ≤5%
儲存溫度
−20°C
一般說明
磷酸化酶激酶包含四个亚基,每个亚基含有α、β、γ以及sigma成分。该sigma成分结合4个钙分子,称为钙调蛋白,而其γ单位可用作催化亚基。
應用
来自兔肌肉的磷酸化酶激酶已应用于一项研究,以评估糖原磷酸化酶的特征。 此外,它还应用于研究肌动蛋白对不同形式肌磷酸化酶激酶的活化作用。
單位定義
在30°C、pH 7.7和ATP存在的条件下,一个单位每分钟可将磷酸化酶B转化为1.0 μ摩尔磷酸化酶。
外觀
含有(NH4)2SO4、蔗糖、β-甘油磷酸盐和二硫赤藓糖醇的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Biological chemistry Hoppe-Seyler, 367(2), 109-117 (1986-02-01)
The activities of glycogen phosphorylases a and b from the body wall musculature of the marine worm Arenicola marina (Annelida, Polychaeta) were determined after various periods of anoxia. Already under normoxic conditions one third of the total activity was produced
Journal of molecular biology, 425(12), 2083-2099 (2013-04-02)
The endoplasmic reticulum transmembrane receptor Ire1 senses over-accumulation of unfolded proteins in the endoplasmic reticulum and initiates the unfolded protein response (UPR). The cytoplasmic portion of Ire1 has a protein kinase domain (KD) and a kinase extension nuclease (KEN) domain
Advances in experimental medicine and biology, 740, 703-730 (2012-03-29)
Calcium/calmodulin-stimulated protein kinases can be classified as one of two types - restricted or multifunctional. This family of kinases contains several structural similarities: all possess a calmodulin binding motif and an autoinhibitory region. In addition, all of the calcium/calmodulin-stimulated protein
Antisense suppression of the small chloroplast protein CP12 in tobacco: a transcriptional viewpoint.
Plant signaling & behavior, 6(12), 2026-2030 (2011-11-25)
The chloroplast protein CP12 forms a multi-enzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and NADP-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12.
Oncogene, 31(39), 4333-4342 (2011-12-20)
Angiogenesis is essential for development and tumor progression. With the aim of identifying new compound inhibitors of the angiogenesis process, we used an established enhanced green fluorescent protein-transgenic zebrafish line to develop an automated assay that enables high-throughput screening of
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