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一般說明
马铃薯酸性磷酸酶是一种磷酸单酯酶,具有多种分子形式,分子量相近,但等电点不同。
應用
检测了马铃薯酸性磷酸酶在各种表面活性剂介质中的活性。 双(2-乙基己基)磺基琥珀酸钠,Brij 35和3mM的SDS抑制磷酸酶活性,而表面活性剂十六烷基三甲基溴化铵增强活性。
马铃薯酸性磷酸酶已用于评估新基因产物的潜在过敏性的研究中。 在一项研究中,它还用于在pH 7.0下从酪蛋白中去除8个磷酸基团。
生化/生理作用
酸性磷酸酶(APase)是一个酶家族,在pH 4-7最佳条件下,非特异性地催化磷酸单酯和酸酐水解产生无机磷酸盐。
單位定義
在pH 4.8 ,37℃ 下,一单位每分钟可水解 1.0 μmole的对-硝基苯基磷酸酯。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)
The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
Archives of biochemistry and biophysics, 288(2), 621-633 (1991-08-01)
An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated.
Regulatory toxicology and pharmacology : RTP, 63(2), 181-187 (2012-04-17)
With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three
Trends in biochemical sciences, 17(3), 105-110 (1992-03-01)
Formation of phosphate esters by kinases has long been recognized as an important process in biochemistry, but the reverse reaction, hydrolysis of phosphate esters by phosphatases, has attracted less attention. Recent work suggests that phosphatases are as important as kinases
实验方案
酸性磷酸酶的酶促测定(EC 3.1.3.2)
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