推荐产品
生物源
mouse
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
RMO44, monoclonal
形狀
buffered aqueous solution
物種活性
human, mouse, rat, zebrafish
濃度
~2 mg/mL
技術
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extract of SHSy5y human neuroblastoma cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
一般說明
Monoclonal Anti- Neurofilament 160 (mouse IgG1 isotype) is derived from the hybridoma RMO44 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified mid-sized rat neurofilament (NF-M) subunit. Neurofilaments are one of the five major groups of intermediate filaments (Ifs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kDa, 160 kDa and 200 kDa.
Neurofilament 160 (NEFM) is one among the most abundant filaments in post-mitotic neurons.
免疫原
purified mid-sized rat neurofilament (NF-M) subunit.
應用
Monoclonal Anti-Neurofilament 160 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- western blot analysis
- immunohistochemistry
- antibody labelling
生化/生理作用
Neurofilament 160 (NEFM) associates with the dopamine receptor.
Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals. These molecules undergo post-translational modifications, which results in their heterogeneity including different levels of phosphorylation. The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing their interaction with cytoplasmic organelles.
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
nwg
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Neurofilaments at a glance
Journal of Cell Science, 125(14), 3257-3263 (2012)
An in vitro model for studying CNS white matter: functional properties and experimental approaches
F1000Research, 8(1) (2019)
Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab
Journal of Neuroscience Research, 97(2), 202-214 (2019)
Neuronal cytoskeletal alterations in an experimental model of depression
Neuroscience, 3-3 (2004)
The amount of neurofilaments aggregated in the cell body is controlled by their increased sensitivity to trypsin-like proteases
Journal of Cell Science, 117(6), 861-869 (2004)
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