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Merck

MAK562

Sigma-Aldrich

Branched Chain Amino Acid Kit

sufficient for 100 colorimetric tests

别名:

BCAA Assay Kit, BCAA Kit

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About This Item

分類程式碼代碼:
41116104
NACRES:
NA.28

用途

sufficient for 100 colorimetric tests

品質等級

檢測方法

colorimetric

相關疾病

endocrinological disorders, diabetes; neurological disorders; gastrointestinal diseases

儲存溫度

−20°C

一般說明

Leucine, isoleucine and valine, known collectively as branched-chain amino acids (BCAA) are unique amongst the essential amino acids in that they undergo oxidation to a greater extent in the peripheral tissues than in liver1. Concentrations of these amino acids in plasma are altered by moderate and exhausting exercise, by diet and the nutritional status of the individual.2

In this assay, BCAA concentration is determined using a coupled enzyme reaction to catalyze the conversion of a BCAA molecule to its ketone form, which results in the conversion of NAD+ to NADH.3 NADH is detected by a colorimetric indicator, with an absorption maximum of 450 nm, proportional to the amount of BCAA present.

應用

BCAA Kit has been used to quantify BCAA in various types of samples, such as food, dietary supplements, blood, serum and cells.

特點和優勢

Highly sensitive, simple and high-throughput assay for measuring BCAA concentration.
Detection time: only 30 minutes
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

適合性

Suitable for measuring Branched Chain Amino Acids (BCAA′s) in a variety of biological and organic samples.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


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