MAK525

Acid Phosphatase Fluorometric Assay Kit

sufficient for 100 fluorometric tests

• 分類程式碼代碼: 12161503

属性

• 品質等級: 100
• 檢測方法: fluorometric
• 相關疾病: genitourinary disorders; cancer; gastrointestinal diseases
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

特點和優勢

• Linear detection range: 0.008 to 10 U/L .
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).

原則

The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H319
• 防範說明: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危險分類: Eye Irrit. 2
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1