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Merck
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主要文件

MAK506

Sigma-Aldrich

Peroxidase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

别名:

Peroxidase Enzyme Activity Kit

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.84

品質等級

檢測方法

colorimetric
fluorometric

相關疾病

cancer; cardiovascular diseases; neurological disorders

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Peroxidase catalyze the following oxidationreduction reactions: ROOR′ + electron donor (2 e-) + 2H+ → ROH + R′OH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi, and bacteria (cytochrome-c peroxidase like enzymes such as horseradish peroxidase).

特點和優勢

  • Linear detection range: 2 to 50 U/L for the colorimetric assay and 0.1 to 5 U/L for the fluorometric assay.
  • Compatible with high-throughput handling systems.

適合性

The kit is suitable for peroxidase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

原則

The Peroxidase Assay Kit uses H2O2 and an electron donor dye that forms resorufin during the peroxidase reaction. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is a direct measure of the enzyme activity.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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