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Merck

MAK486

Sigma-Aldrich

Carbonyl Assay Kit

sufficient for 100 colorimetric tests

别名:

Protein Carbonyl Content Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

檢測方法

colorimetric

相關疾病

cardiovascular diseases; cancer; gastrointestinal diseases; aging/geriatric diseases; neurological disorders

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Carbonyl groups, such as ketones and aldehydes, are a common indicator of protein oxidation. Protein oxidation is caused because of exposure to reactive oxygen species (ROS) and is a common marker in various diseases, as well as aging.

特點和優勢

  • Linear detection range from 12 to 250 µM carbonyl
  • Compatible with high-throughput handling systems.
  • Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes.

適合性

The kit is suitable for the quantification of carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc.).

原則

The Carbonyl Assay Kit is based on an improved method, where 2,4-dinitrophenylhydrazine (DNPH) reacts with carbonyl groups to produce a colored compound at 375 nm. The intensity of this colored compound is directly proportional to the carbonyl groups in the sample.

象形圖

Corrosion

訊號詞

Warning

危險聲明

防範說明

危險分類

Met. Corr. 1

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 1


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