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Merck

MAK162

Sigma-Aldrich

马来酰亚胺检测试剂盒(比色法)

sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

相關疾病

cancer

儲存溫度

−20°C

一般說明

Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.

適合性

Suitable for quantifying maleimide groups

原則

The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.

象形圖

Exclamation mark

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


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Carbon Composites: Composites with Carbon Fibers, Nanofibers, and Nanotubes (2016)
Efficient site-specific labeling of proteins via cysteines.
Kim Y, et al.
Bioconjugate Chemistry, 19(3), 786-791 (2008)

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