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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
cancer
儲存溫度
−20°C
一般說明
Maleimide is an unsaturated imide used in protein conjugation reactions. The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.
適合性
Suitable for quantifying maleimide groups
原則
The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
閃點(°F)
188.6 °F - closed cup
閃點(°C)
87 °C - closed cup
Carbon Composites: Composites with Carbon Fibers, Nanofibers, and Nanotubes (2016)
Efficient site-specific labeling of proteins via cysteines.
Bioconjugate Chemistry, 19(3), 786-791 (2008)
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