推荐产品
![2-脱氧-2-[（7-硝基-2,1,3-苯并恶二唑-4-基）氨基]-D-葡萄糖 ≥97% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/104/527/40bd5a41-ebc4-484e-a10e-891fecfaea79/640/40bd5a41-ebc4-484e-a10e-891fecfaea79.png)
用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
endocrinological disorders, diabetes; cancer
儲存溫度
−20°C
一般說明
葡萄糖是大多数细胞的主要能量来源。葡萄糖的细胞摄取是葡萄糖代谢的首个限速步骤,该过程受到严密的调控。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素耐受性细胞在遇到胰岛素刺激时,葡萄糖摄取会减弱。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
使用再循环扩增反应比色法（20-100 pmol）,检测限要比荧光法检测（200-1000 pmol,MAK084) 低10倍。
應用
葡萄糖摄取检测试剂盒（比色法）已被用于测量各种细胞的葡萄糖摄取。
適合性
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
原則
葡萄糖摄取检测试剂盒（比色法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中2-DG6P在此过程中被氧化生成NADPH，随后通过再循环扩增反应来测定NAPDH浓度,而后一反应中谷胱甘肽还原酶将利用NADPH生成谷胱甘肽。谷胱甘肽产物与DTNB反应生成TNB，后者可在412 nm波长处检测到。
Heng-Jing Hu et al.
Frontiers in pharmacology, 12, 690371-690371 (2021-12-25)
Atrial fibrosis is the basis for the occurrence and development of atrial fibrillation (AF) and is closely related to the Warburg effect, endoplasmic reticulum stress (ERS) and mitochondrion dysfunctions-induced cardiomyocyte apoptosis. Hydrogen sulfide (H2S) is a gaseous signalling molecule with
Yu-Yong Wang et al.
Disease markers, 2022, 9516774-9516774 (2022-05-17)
Prostate cancer (PCa) is one of the most common malignancies in men with high death rate worldwide. Paclitaxel (Taxol) is a widely used anticancer agent. Despite recent improvements in clinical application and research, development of drug resistance limits the efficacy
PLIN2 inhibits insulin-induced glucose uptake in myoblasts through the activation of the NLRP3 inflammasome.
Cho K A and Peter B K
International Journal of Molecular Medicine, 36(3), 839-844 (2015)
Méndez-Martínez Marisol et al.
Scientific reports, 9(1), 13372-13372 (2019-09-19)
Obesity is caused by lipid accumulation in adipose tissues inducing adipocyte dysfunction, characterized by insulin resistance, increased lipolysis, oxidative stress, and inflammation, leading to increased levels of adipokines. Herein the capacity of the subfractions (SFs) SF1, SF2, and SF3 from
MicroRNA-138 suppresses proliferation, invasion and glycolysis in malignant melanoma cells by targeting HIF-1α.
Chen Y, et al.
Experimental and Therapeutic Medicine, 11(6), 2513-2518 (2016)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门