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Merck

MAK070

Sigma-Aldrich

草酰乙酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

檢測方法

colorimetric
fluorometric

相關疾病

cancer

儲存溫度

−20°C

一般說明

草酰乙酸 (OAA) 是三羧酸循环和糖异生的中间产物。在柠檬酸盐之前,通过柠檬酸盐合成酶介导的乙酰辅酶 a 和 OAA 缩合形成柠檬酸盐。OAA 也可由天冬氨酸脱酰胺或 CO 2 与丙酮酸或 PEP 缩合形成。由于哺乳动物不具备从乙酰辅酶 a 形成三羧酸循环中间产物的酶促机制,因此 OAA 是通过丙酮酸和丙酮酸羧激酶的回补进入点之一。条目草酰乙酸测定试剂盒提供了一种简单、灵敏和快速的方法,可定量测定多种样品中的 OAA。

應用

草酰乙酸测定试剂盒用于测定样品中草酰乙酸的浓度。

適合性

适用于动物组织、细胞培养(贴壁细胞或悬浮细胞)、血清和唾液。

原則

在本试剂盒中,通过偶联酶试验测定草酰乙酸盐浓度,得到比色 (570 nm)/荧光 (lex = 535/lem = 587 nm) 产物,与存在的草酰乙酸成比例。该试剂盒的典型检测范围为 2-10 nmole(比色法)和 0.2-1 nmole(荧光法)。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids


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The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
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Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how the hypoxia regulates tumor cells remains unclear. Here, we show that hypoxia promotes the growth of human breast tumorigenic cells that repopulate tumors
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
Bartok O, et al.
The Embo Journal, 34(11), 1538-1553 (2015)

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