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Merck

MAK039

Sigma-Aldrich

乙酰辅酶A检测试剂盒

sufficient for 100 fluorometric tests

别名:

乙酰辅酶A检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 100 fluorometric tests

檢測方法

fluorometric

相關疾病

cancer; neurological disorders

儲存溫度

−20°C

一般說明

乙酰辅酶A是酶促的乙酰转移反应中酰基的必需辅助因子和载体。它可通过线粒体中丙酮酸的氧化脱羧、长链脂肪酸的氧化或某些特定氨基酸的氧化降解而形成。乙酰辅酶A是柠檬酸循环(克雷布氏循环)的起始化合物。它也是脂质生物合成的关键前体,所有脂肪酸碳的来源。乙酰辅酶A正向调节丙酮酸羧化酶活性。它是神经递质乙酰胆碱的前体。组蛋白乙酰基转移酶(HAT)在组蛋白和非组蛋白翻译后乙酰反应中使用乙酰辅酶A作为乙酰基供体。

乙酰辅酶A是脂肪,糖和蛋白质水平的指示剂,表明营养水平。因此,饥饿能够很大程度地降低乙酰辅酶A水平,激活自吞噬过程。在低葡萄糖条件下,乙酰辅酶A参与ATP生成。因为脂肪酸利用升高,在前列腺癌中观察到乙酰辅酶A水平升高,为肿瘤细胞生长提高额外能量。营养不良会引起某些代谢物水平改变,包括乙酰辅酶A,激活表观遗传修饰,影响中枢神经系统。

應用

乙酰辅酶A试剂盒可以用来定量暴露于重组HIV蛋白Tat和可卡因的胶细胞中乙酰辅酶A水平。它还可以用于通过缺氧在原代人心室肌细胞引发压力,提高乙酰辅酶A水平,然后研究线粒体代谢的研究。

適合性

适用于检测多种生物样品中的乙酰辅酶A。

原則

乙酰辅酶A浓度能够被偶联酶分析检测,生成的荧光 (λex = 535/λem = 587 nm)产物和乙酰辅酶A成正比。该试剂盒通常的检测灵敏度是10-1000pmole的乙酰辅酶A。该试剂盒检测各种生物样本中的乙酰辅酶A的灵敏度都很高。

相關產品

产品编号
说明
价格

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

閃點(°F)

188.6 °F - closed cup

閃點(°C)

87 °C - closed cup


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