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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
endocrinological disorders, diabetes; gastrointestinal diseases
儲存溫度
−20°C
基因資訊
human ... AMY1A(278)
一般說明
淀粉酶是一种糖苷水解酶，它能裂解多糖(如淀粉或糖原)中的葡聚糖键，从而释放多糖分子。α-淀粉酶在动物、植物、和许多真菌中均有发现。在哺乳动物中，α-淀粉酶是淀粉酶的主要形式，是饮食消化的主要酶之一。α淀粉酶的活性和稳定性都依赖于钙离子。在人类中，α-淀粉酶的水平在胰腺和唾液分泌物中最高。血液和尿液淀粉酶水平的升高是由于胰腺疾病和唾液腺损伤引起的。唾液淀粉酶活性的增加也是交感神经系统活性增加的一个标志。
應用
采用淀粉酶活性测定试剂盒已可用于测定淀粉酶的活性。
適合性
适用于定量测定各种生物样品中的淀粉酶活性。
原則
淀粉酶活性测定试剂盒可定量测定多种生物样品中的淀粉酶活性。该试剂盒快速，方便并且灵敏。在该试剂盒中，淀粉酶活性是通过耦合酶法测定的，其产生一个比色(405 nm)的产品，与底物的数量成比例的乙烯-pNP-G7，由淀粉酶裂解得到。一个单位的淀粉酶，在25℃下，每分钟可裂解乙烯-pNP-G7而产生1.0 μmole的 p-硝基酚。
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商品
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
Assay protocol for the colorimetricdetection of amylase activity in biological samples using the Amylase Activity assay kit.
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