推荐产品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 80-85 kDa
物種活性
rat, human, mouse
包裝
antibody small pack of 25 μL
濃度
~1 mg/mL
技術
immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates
western blot: 0.5-1 μg/mL using Jurkat cell lysates
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MLH1(4292)
mouse ... Mlh1(17350)
rat ... Mlh1(81685)
一般說明
MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.
MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).
免疫原
synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.
應用
- Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化／生理作用
MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.
MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).
標靶描述
MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome
Steinke V. et al.
Deutsches Arzteblatt International, 110(3), 32-32 (2013)
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
Wang Y, et al.
Genes & Development, 14(8), 927-939 (2000)
Association between MutL homolog 1 polymorphisms and the risk of colorectal cancer: a meta-analysis.
Haiyan Chen et al.
Journal of cancer research and clinical oncology, 141(12), 2147-2158 (2015-05-20)
As one of the most essential components of mismatch repair system, MutL homolog 1 (MLH1) plays an increasingly implicated role in initiation and promotion of colorectal carcinogenesis, with germ-line mutations in different loci. However, whether a single genetic variant in
Michael S Landau et al.
Human pathology, 45(8), 1704-1712 (2014-06-09)
Reduced CDX2 and cytokeratin 20 (CK20) expression in colorectal carcinoma with BRAF mutation and high-level microsatellite instability (MSI-H) has been well documented. The immunophenotype of BRAF-mutated microsatellite stable (MSS) colorectal carcinoma has not been reported. We analyzed 205 colorectal carcinomas
Koah R Vierkoetter et al.
Gynecologic oncology, 135(1), 81-84 (2014-08-06)
Patients with Lynch Syndrome are at an increased risk for a variety of malignancies, including ovarian cancer. Ovarian cancers associated with Lynch Syndrome are predominantly clear cell or endometrioid in histology. Lynch Syndrome is characterized by germline mutations in mismatch
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