推荐产品
产品名称
Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal, clone ERK-NP2, purified from hybridoma cell culture
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
ERK-NP2, monoclonal
形狀
buffered aqueous solution
分子量
antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa
物種活性
human, rat
濃度
~2 mg/mL
技術
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-25 μg/mL using rat brain extract
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MAPK1(5594) , MAPK3(5595)
rat ... Mapk1(116590) , Mapk3(50689)
一般說明
Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
特異性
The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2).
免疫原
synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.
應用
A working concentration of 5-25 μg/mL may be used for detection by immunoblotting in rat brain extract. The antibody has also been used at working dilution of 1:50 for MAPK quantification by flow cytometry in monocyte-derived macrophages (MDM) derived from Holstein cattle.
外觀
Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
準備報告
Prepared from a culture supernatant of bioreactor grown hybridoma
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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