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一般說明
来自 双花扁豆(硬皮豆)的凝集素是一种异源四聚体糖蛋白,其分子量约为113 kDa。双花扁豆凝集素(DBA)对于末端α连接的N-乙酰基-D-半乳糖胺(GalNAc)有特异性。它是一种膳食凝集素。
應用
来自双花扁豆(硬皮豆)的凝集素已被用于:
- 表征小鼠子宫自然杀伤(uNK)细胞
- 采用凝集素双重染色定位核因子-κB(NF-κB)活化
- 作为物种特异性生殖母细胞标记物用于确定供体细胞定植
- 标记未分化的精原细胞/猫睾丸组织
生化/生理作用
双花扁豆凝集素(DBA)具有结合活性,可用作双核细胞的标记物,并可指示其分化状态。它可用作原始生殖细胞(PGC)、生殖母细胞、前精原细胞和精原细胞的标记物。
DBA具有抗人-A1血型特异性,可用于区分A1和A2血型。 DBA具有很强的抗-Cad活性,并且对末端N-乙酰基-α-D-半乳糖胺基残基具有亲和力。从亲和纯化的凝集素制备偶联物。
包裝
按活性结合物毫克数出售。
外觀
pH 7.5,含 10 mM HEPES、0.15 M NaCl、0.08% 叠氮化钠、0.1 mM Ca2+
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
Analysis of uterine natural killer cells in mice
Natural Killer Cell Protocols, 465-503 (2010)
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Andrology, 4(6), 1159-1168 (2016)
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