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生物源
fungus (Trichoderma viride)
品質等級
形狀
lyophilized powder
比活性
≥20 units/mg protein
分子量
112 kDa
成份
Protein, 5-20%
儲存溫度
2-8°C
一般說明
赖氨酸氧化酶(来自绿色木霉)是一种分子量为112kDa的同型二聚体黄素酶。在65°C下稳定,对L-赖氨酸具有高度选择性。它由FAD结合、底物结合和螺旋结构域组成,具有明显的活性部位通道。
應用
赖氨酸氧化酶(来自绿色木霉)已用于制备生物发光芯片。
生化/生理作用
赖氨酸氧化酶(来自绿色木霉)通过催化L-赖氨酸的氧化脱氨形成α-酮基-ε-氨基己酸。它在白血病细胞中表现抗癌能力,也是鳞状细胞、成纤维细胞、卵巢和胃癌的肿瘤抑制剂。赖氨酸氧化酶在结缔组织结构完整性和胚胎发育中起重要作用。
單位定義
一个单元在 37°C,pH 8.0 的条件下催化 L-赖氨酸每分钟生成 1 μmol 的 6-氨基-2-氧代己酸。
外觀
含有磷酸盐缓冲盐和稳定剂
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Design of luminescent biochips based on enzyme, antibody, or DNA composite layers
Analytical and Bioanalytical Chemistry, 377(5), 922-928 (2003)
Voprosy meditsinskoi khimii, 44(4), 384-387 (1998-12-10)
L-lysin-a-oxidase (LO), a fungal enzyme catalysing oxidative deamination of L-lysin, was used for the inhibition of the reproduction of herpes simplex virus type 1 (HSV-1). Antiviral activity of LO was tested in vitro. The expression of viral antigens and CPE
Bioelectrochemistry (Amsterdam, Netherlands), 75(2), 124-129 (2009-04-07)
Immobilization of L-lysine alpha-oxidase on gold-mercaptopropionic acid self-assembled monolayer (Au-MPA-LOx SAM) electrode is verified experimentally in the present work. Fabrication steps and electrochemical interaction of Au-MPA-LOx with L-lysine were monitored by general electrochemical methods like cyclic voltammetry (CV) and chronoamperometry
Studies on Anti-Cancer Activity of Lysyl Oxidase from Trichoderma Viride MTCC 167
International Journal of Applied Sciences and Biotechnology, 4(1), 57-63 (2016)
Biosensors & bioelectronics, 35(1), 439-442 (2012-03-16)
An amperometric biosensor was proposed for the enantioanalysis of L-lysine. The biosensor is based on the impregnation of L-lysine oxidase in diamond paste. The potential used for the determination of l-lysine was 650 mV. The biosensor exhibited a linear concentration
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