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Key Documents

HSANGERV

Sigma-Aldrich

桑格全基因组 慢病毒 CRISPR 文库

Human, Virus Format

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About This Item

分類程式碼代碼:
41105904

品質等級

200

包裝

pkg of 10 μL (384-well plate)

濃度

1x106  VP/ml (via p24 assay)

應用

CRISPR

運輸包裝

dry ice

儲存溫度

−70°C

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一般說明

两名基因组编辑领导者Sigma-Aldrich和Wellcome Trust Sanger Institute联手打造了第一个阵列化的慢病毒CRISPR基因敲除文库。基于文献中已验证的技术，Sanger CRISPR库将使您的实验室走在竞争的最前沿，以实现下一个重大发现。

應用

功能基因组学/筛选/靶标验证

特點和優勢

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

包裝

384-well plates

成分

Lentivirus Transduction Particles of gRNA-only lenti-based vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones.


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外觀

10 μl of lentivirus at ≥106 particles/ml in 102×384-well plates

其他說明

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

推薦產品

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Mouse, Virus Format

法律資訊

CRISPR Use License Agreement

Lentiviral License Agreement

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)

商品

Successful Transduction Using Lentivirus

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

实验方案

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

FACS Titration of Lentivirus Protocol

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

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