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GE28-9852-56

rProtein A/Protein G GraviTrap™

Cytiva 28-9852-56, pack of 10 × 1 mL

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

包裝

pack of 10 × 1 mL

製造商／商標名

Cytiva 28-9852-56

儲存條件

(20% Ethanol)

床體積

1 mL

容量

35 mg binding capacity (human IgG)(Protein binding capacity is protein-to-protein dependent.)

儲存溫度

2-8°C

一般說明

rProtein A Protein G GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of a mixture of 50% rProtein A Sepharose™ Fast FLow and 50% protein G Sepharose™ 4 Fast Flow.

應用

rProtein A Protein G GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A and Protein G ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

特點和優勢

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as Protein A/Protein G GraviTrap™ ~ 35 mg human IgG/column.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare.

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

实验方案

Performing a Purification of IgG Antibodies with Protein G GraviTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from GE Healthcare

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