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ligand
diethylaminoethyl
描述
Ion Exchanger Type (value)
形狀
prepacked column
交聯
cross-linked
包裝
pkg of 1 ea
製造商/商標名
Cytiva 28-9782-45
儲存條件
(20% Ethanol)
參數
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
技術
liquid chromatography (LC): suitable
床尺寸
7.7 mm × 100 mm
床體積
4.7 mL
柱內徑
7.7 mm
基質
6% cross-linked agarose
基質活性組
N+(C2H5)2H functional group
粒徑
45-165 μm
平均直徑
90 μm
cleaning
2-14
工作範圍
2-12
容量
binding capacity(~ 110 mg HAS/mL resin)
適合性
suitable for bioprocess medium
分離技術
anion exchange
一般說明
應用
If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
特點和優勢
- DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
实验方案
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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