推荐产品
ligand
protein G
包裝
pack of 5 × 1 mL
製造商/商標名
Cytiva 28-9670-70
儲存條件
(20% Ethanol, 10% medium slurry)
參數
Room temperature temp. range
基質
paramagnetic, spherical, highly cross-linked agarose particles
容量
27 mg binding capacity(human IgG/ml gel)
儲存溫度
2-8°C
一般說明
應用
Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.
Protein G Mag Sepharose™ is another product in the Mag Sepharose™ platform which is designed for the rapid capture and enrichment of Protein samples from cell lysates and biological fluids using immunoprecipitation technology. Protein G Mag Sepharose™ is made with an optimized ligand for immunoprecipitation.
特點和優勢
- Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
- High purity and yield
- Robust parallel screening of antibodies with high reproducibility
- Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
- Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
实验方案
This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.
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