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GE28-9670-66

Protein G Mag Sepharose™ Xtra

Cytiva 28-9670-66, pack of 2 × 1 mL

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

protein G

儲存期限

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包裝

pack of 2 × 1 mL

製造商／商標名

Cytiva 28-9670-66

儲存條件

(20% Ethanol, 10% medium slurry)

參數

Room temperature temp. range

基質

paramagnetic, spherical, highly cross-linked agarose particles

容量

27 mg binding capacity(human IgG/ml gel)

儲存溫度

2-8°C

相关类别

一般說明

protein G Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG /mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

應用

Protein G Mag Sepharose™ Xtra magnetic beads combine proven affinity chromatography methods with the magnetic bead platform to improve simplification and ease-of-use in sample preparation. Mag Sepharose™ beads are dense, black and visible, and this makes them simple to handle. Less smearing effects and aggregate formations of the magnetic beads mean that no detergents are needed in the buffers.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device. Protein G Mag Sepharose™ Xtra is available in a 2 × 1 mL pack for 20 purifications and in a 5 × 1 mL pack for 50 purifications. The product is based on Sepharose™ medium with magnetite incorporated and the ligand is native Protein G. Protein G has high affinity for the Fc region of IgG antibody from a variety of species.

特點和優勢

  • Efficient, high capacity small-scale purification and screening of antibodies from various species; 27 μg human IgG/μL magnetic beads
  • High purity and yield
  • Robust parallel screening of antibodies with high reproducibility
  • Simple capture of antibodies from small or large sample volumes (low-microliter to high-milliliter scale)
  • Visible and dense Sepharose™ based magnetic beads give increased simplicity for handling
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

实验方案

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

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