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Key Documents

GE28-9031-34

Protein G High Performance Spintrap™

Cytiva 28-9031-34, pack of 16 columns

别名:

Protein G Column

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

特點

wetted part: no

包裝

pack of 16 columns

製造商／商標名

Cytiva 28-9031-34

儲存條件

(20% Ehtanol)
 (20% Ethanol)

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

2-9

工作範圍

3-9

容量

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 >1 mg binding capacity(human IgG/column)

儲存溫度

2-8°C

一般說明

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein G HP SpinTrap™ is a prepacked, single-use spin column containing protein G Sepharose™ High Performance.

應用

Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

特點和優勢

  • For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

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商品

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

实验方案

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from GE Healthcare.

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