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形狀
liquid
特點
wetted part: no
包裝
pkg of 1 mL
製造商／商標名
Cytiva 17-6003-18
儲存條件
(Rehydration Solution -20)
顏色
Colorless
儲存溫度
2-8°C
一般說明
DeStreak™ Reagent.
應用
The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7.0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsen the problem. Extra spots on 2-D gels, caused by nonspecific oxidation of Proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and nonspecific oxidation result in poorly resolved Protein patterns and reduced reproducibility between electrophoresis runs.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
DeStreak™ Rehydration Solution effectively eliminates nonspecific oxidation by maintaining Protein thiol groups in a single oxidation state, irrespective of sample load, pH range, or run length. Protein patterns are stabilized in IPG strips of any length and pH gradient ensuring the same stable and reproducible pattern in every analysis. The effectiveness of DeStreak™ Rehydration Solution is maintained at the high sample loads and long run times required for preparative gel electrophoresis.
DeStreak™ Rehydration Solution contains optimized concentrations of urea, thiourea, CHAPS, and DeStreak™ Reagent. The solution is ready for use after addition of the appropriate IPG buffer. DeStreak™ Reagent is also available separately in a 1-ml pack size.
特點和優勢
- Improves reproducibility and quality of 2-D gels by preventing streaking.
- Eliminates extra spots caused by nonspecific oxidation of Proteins.
- Stabilizes Proteins throughout entire 2-D runs, ensuring simplified, well-resolved Protein patterns.
- Highly suitable for both analytical and preparative gels.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
DeStreak is a trademark of Cytiva
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 3 Oral - Eye Dam. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
233.6 °F - closed cup
閃點（°C）
112 °C - closed cup
其他客户在看
商品
This page describes components of rehydration solutions for use in 2-D Electrophoresis with GE Healthcare products.
实验方案
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from GE Healthcare.
This page describes typical rehydration solutions for use in gel electrophoresis with GE Healthcare products.
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