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Key Documents

GE17-6002-33

HiTrap® IEX Selection Kit

Cytiva 17-6002-33

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

quaternary amine

描述

Ion Exchanger Type (value)

儲存期限

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

包裝

pkg of 7 ea

製造商/商標名

Cytiva 17-6002-33

參數

42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

2-14

工作範圍

2-12

適合性

suitable for bioprocess medium

相关类别

一般說明

HiTrap® IEX Selection Kit consists of seven HiTrap® columns, prepacked with different Sepharose Fast Flow ion exchange chromatography media for screening of the most appropriate media and conditions to use for specific application and development work.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.Q and SP Sepharose XL media have long chains of dextran coupled to a robust, 6% highly cross-linked agarose matrix. The dextran chains increase the exposure of the Q or SP charged groups, which results in very high loading capacities. The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached. After choosing the optimal medium, prepacked columns and bulk media are available for larger scale preparative work.

特點和優勢

  • Fast and easy selection with seven different ion-exchange ligands on Sepharose Fast Flow and Sepharose XL, prepacked in 1 mL HiTrap® columns.
  • Offers a fast, easy and convenient way screen for the optimal media for a given application .
  • Excellent for optimization studies; buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
  • Designed for use with a syringe, peristaltic pump, or chromatography system such as AKTA design.

儲存和穩定性

Store at 4 to 30 °C (20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP))
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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实验方案

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

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