GE17-5079-01

HiTrap^® rprotein A Fast Flow

Cytiva 17-5079-01, pack of 5 × 1 mL

• 分類程式碼代碼: 23151816
• NACRES: NA.56

属性

• ligand: recombinant protein A (E. coli)
• 包裝: pack of 5 × 1 mL
• 製造商／商標名: Cytiva 17-5079-01
• 儲存條件: (20% Ehtanol); (20% Ethanol)
• 參數: <4 mL/min flow rate; 42 psi
• 床尺寸: 7 mm × 25 mm
• 床體積: 1 mL
• 柱內徑: 7 mm
• 基質: 4% cross-linked agarose
• 粒徑: 60-165 μm
• 平均直徑: 90 μm (d50v)
• cleaning: 2-11
• 工作範圍: 3-10
• 容量: ~50 mg binding capacity (human lgG/ml medium)
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

说明

一般說明

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established protein A-based medium used in many approved MAb processes.
• Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

安全信息

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids