推荐产品
ligand
recombinant protein A (E. coli)
儲存期限
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
包裝
pack of 1 × 5 mL
製造商／商標名
Cytiva 17-5080-01
儲存條件
(20% Ehtanol)
(20% Ethanol)
參數
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
床尺寸
16 mm × 25 mm
床體積
5 mL
柱內徑
16 mm
基質
4% cross-linked agarose
粒徑
60-165 μm
平均直徑
90 μm (d50v)
cleaning
2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
工作範圍
3-10
容量
~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
相关类别
一般說明
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
特點和優勢
- Quick, convenient preparative purification of monoclonal antibodies.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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