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ligand
octylamine
包裝
pack of 100 mL
製造商／商標名
Cytiva 17-5481-02
基質
highly cross-linked agarose
樣品平均尺寸
75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
3-13(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
工作範圍
2-14(pH interval where the medium can be operated without significant change in function.)
容量
~13 mg binding capacity (ovalbumin)
適合性
suitable for bioprocess medium
一般說明
Capto™ Core 700 exhibits dual functionality due to the novel core bead technology and multimodal, octylamine ligand. This chromatography resin possesses size exclusion and binding properties. Capto™ Core 700 comprises a ligand-activated core and inactive shell. The inactive shell aids in preventing large molecules (cut off ~ Mr 700 000) from entering the core via the pores of the shell. The core of each bead is functionalized with hydrophobic and positively charged ligands. The smaller size impurities bind to the multimodal internalized ligands and the larger molecules get collected in the column flowthrough. Cleaning-in-place (CIP) processes use NaOH and, in most cases, a solvent to remove bound contaminants from the beads. HiTrap® and HiScreen™ columns can be linked in series for enhanced capacity, making them ideal for generating an efficient and robust separation system.
Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
特點和優勢
- This novel core bead technology enables efficient contaminant capture while target molecules get trapped in the flowthrough.
- Hydrophobic and positively charged ligands
- Offers improved productivity and higher flow rates than standard size exclusion (gel filtration) methods.
- Flowthrough chromatography allows straightforward and robust performance.
- Multimodal removal of contaminants
- Convenient small-scale purification and process development
- Scaling up is simple using prepacked HiTrap® and HiScreen™ columns.
- Resin meets industrial demands for supply security and regulatory support.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Capto is a trademark of Cytiva
HiScreen is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page describes the maintenance of media and storage conditions for multimodal chromatography using GE Healthcare products.
实验方案
Capto Core 700 from GE Healthcare is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
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