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ligand
octylamine
包裝
pack of 25 mL
製造商／商標名
Cytiva 17-5481-01
基質
highly cross-linked agarose
樣品平均尺寸
75 (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning in place
3-13(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
工作範圍
2-14(pH interval where the medium can be operated without significant change in function.)
容量
~13 mg binding capacity (ovalbumin)
適合性
suitable for bioprocess medium
一般說明
Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.
Novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality. This chromatography resin has both size exclusion and binding properties.
Multimodal removal of contaminants
Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
Hydrophobic, positively charged ligands
The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
Scaling up is simple
HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.
Multimodal removal of contaminants
Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
Hydrophobic, positively charged ligands
The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.
Scaling up is simple
HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.
應用
- Multimodal contaminate removal
- Virus and exosome purification
- Process development
- Biomolecule purification
特點和優勢
- Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
- Significantly improved productivity and higher flow rates compared with standard size exclusion (gel filtration) methods.
- Straightforward optimization due to flowthrough chromatography and robust performance.
- Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
- Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Capto is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes the maintenance of media and storage conditions for multimodal chromatography using GE Healthcare products.
实验方案
Capto Core 700 from GE Healthcare is designed for intermediate purification and polishing of viruses and other large biomolecules in flowthrough mode.
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