推荐产品
ligand
Cibacron Blue
包裝
pack of 50 mL
製造商/商標名
Cytiva 17-0948-01
儲存條件
(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning in place
3-13
工作範圍
4-12
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
一般說明
Blue Sepharose™ 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Blue Sepharose™ 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.
Blue Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特點和優勢
- Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
- BioProcess medium supported for industrial applications and well-established in approved processes
- Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
This page shows how to remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from GE Healthcare.
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose 4B, HiTrap Blue HP and Blue Sepharose 6 Fast Flow
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