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形狀
powder
技術
activity assay: suitable
基質
cross-linked dextran
基質活性組
functional group
珠尺寸
40-125 μm (dry)
孔徑
~30,000 Da exclusion limit
工作pH值
2-9
容量
3-4 meq/g ion exchange capacity
相容性
mode of use mixed weak and strong anion exchange chromatography
一般說明
Sephadex® 离子交换剂含有具有官能团的交联葡聚糖基质。这些基团通过稳定的醚键连接到基质中的葡萄糖单元。DEAE Sephadex 可用于离子交换色谱法纯化和分离蛋白质。
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
A25120-100G′s 更新货号为 GE17-0170-01
A25120-500G′s 更新货号为 GE170180-02
應用
DEAE® Sephadex A-25 已用于:
- 形成 DEAE Sephadex A-25(甲酸盐形式),用于使用辣根植物提取硫代葡萄糖苷(GLS)
- 甘蓝芽中 GLS 含量的测定
- 星形胶质细胞制备和纯化恩多泽平
- 在急性心肌梗死(AMI)患者中鉴定和标记胰岛素激活的一氧化氮合酶抑制剂(蛋白)
特點和優勢
- 弱阴离子交换剂
- 适用于批处理技术
- 高结合能力
- 对常用的水性缓冲剂添加剂(如 8M 尿素和 6M 盐酸胍)无反应
法律資訊
DEAE Sephadex is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle.
Experimental cell research, 139(2), 401-404 (1982-06-01)
Neuroscience, 46(4), 793-806 (1992-01-01)
We previously isolated two glutamate-binding proteins from rat brain synaptic membranes of 71,000 and 63,000 mol. wt [Chen et al. (1988) J. biol. Chem. 263, 417-426]. In the present study, the 71,000 and 63,000 mol. wt glutamate-binding proteins were purified
The Journal of biological chemistry, 270(38), 22271-22276 (1995-09-22)
Epitopes for monoclonal antibodies directed against the purified adult rat skeletal muscle sodium channel (rSkM1) were localized using channel proteolysis and fusion proteins. The interactions between these and other monoclonal antibodies with site-specific polyclonal antibodies were used to investigate the
Isolation and study of insulin activated nitric oxide synthase inhibitory protein in acute myocardial infarction subjects.
Journal of Thrombosis and Haemostasis, 33(3), 218-229 (2012)
Human Lymphocyte Agglutinins in <I>Tursiops Truncatus</I> (Bottlenose Dolphin).
Immunological Investigations, 12(5), 481-490 (1983)
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