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Key Documents

GE17-0430-01

CNBr Activated Sepharose™ 4B

Cytiva 17-0430-01, pack of 15 g

别名:

CNBr Sepharose 4B

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

包裝

pack of 15 g

製造商／商標名

Cytiva 17-0430-01

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

ligand dependent

2-11

儲存溫度

2-8°C

一般說明

CNBr-activated Sepharose™ 4B is a pre-activated media used for coupling antibodies or other large proteins containing -NH? groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.

CNBr-activated Sepharose™ 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to carry out. The application area covers immobilization of proteins, peptides and nucleic acids.

特點和優勢

  • For immobilizing antibodies or other large proteins containing -NH2 groups by coupling them to the matrix
  • Over 25 years proven track record for coupling ligands containing primary amino groups
  • Multipoint attachment resulting in a chemically stable product
  • The coupling reaction by the cyanogen bromide method is spontaneous, rapid and easy to carry out

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

儲存類別代碼

13 - Non Combustible Solids

分析证书(COA)

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Arina L Maltseva et al.
Frontiers in physiology, 5, 497-497 (2015-01-08)
Immune responses of invertebrate animals are mediated through innate mechanisms, among which production of antimicrobial peptides play an important role. Although evolutionary Polychaetes represent an interesting group closely related to a putative common ancestor of other coelomates, their immune mechanisms

实验方案

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

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