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Key Documents

E6754

Sigma-Aldrich

环氧基-活化-琼脂糖凝胶 6B

lyophilized powder

别名:

环氧活化琼脂糖

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About This Item

MDL號碼:
分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

lyophilized powder

標籤範圍

19-40 μmol per mL

技術

affinity chromatography: suitable

基質

Sepharose 6B

基質活性組

epoxy

基質結合

through epoxy to hydroxyl

基質墊片

12 atoms

膨潤

1 g swells to 3.0-5.0 mL

粒徑

45—165 μm

儲存溫度

2-8°C

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一般說明

when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

應用

环氧活化琼脂糖凝胶用于亲和层析、蛋白层析和活化/功能化基质。环氧活化琼脂糖凝胶已用于获得抗原核表达的口蹄疫病毒 (FMDV) 非结构蛋白 (NSP) 3B 的单克隆抗体,以及研究 B 脑膜炎球菌病。

其他說明

配体:1,4-双(2:3-环氧丙氧基)丁烷
连接:一个环氧基
间隔基:当配体与游离环氧基偶联时,提供 12 原子间隔基。连接是一种稳定的不带电的醚。

外觀

冻干粉末

法律資訊

Sepharose is a trademark of Cytiva

取代透過

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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C A O'Brian et al.
Cancer research, 48(13), 3626-3629 (1988-07-01)
We have previously demonstrated that tamoxifen and related triphenylethylene compounds are potent inhibitors of protein kinase C (PKC). The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose
Dong Li et al.
Veterinary research communications, 34(5), 445-457 (2010-06-01)
A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then
Adam Frankel et al.
RNA (New York, N.Y.), 9(7), 780-786 (2003-06-18)
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino
Kasim Abass Askar et al.
Applied biochemistry and biotechnology, 165(1), 336-346 (2011-04-19)
The purpose of this study was to develop a protocol for the purification of acetylcholinesterase (AChE, acetylcholine acetylhydrolase, E.C.3.1.1.7) enzyme and to extend a purification method for further enzyme characterization. A further aim was to study whether the edrophonium's pharmacologic
A N Jina et al.
Biochemistry, 29(21), 5203-5209 (1990-05-29)
To synthesize an affinity matrix for isolation of D-myo-inositol 1,4,5-trisphosphate binding proteins, racemic 3-cyclohexene-1-carboxaldehyde was oxidized and converted to a mixture of trans-3,4-di-hydroxycyclohexane-1-carboxylic acid methyl ester isomers, which was phosphorylated and separated into (+-)-(1R,3R,4R)- and (+-)-(1R,3S,4S)-trans-3,4-bis[(diphenoxyphosphoryl)oxy]cyclohex an e-1- carboxylic acid

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