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Merck

G2500

Sigma-Aldrich

明胶 来源于猪皮肤

gel strength 300, Type A

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.61

生物源

Porcine skin

品質等級

種類

Type A

化驗

70-90% (biuret)

形狀

powder

技術

cell culture | mammalian: suitable

凝膠強度

~300 g Bloom

溶解度

H2O: soluble 50 mg/mL

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一般說明

明胶是一种亲水胶体。商业生产的明胶来自于牛骨、猪骨和兽皮。作为胶原部分水解产物,主要成分为蛋白质,其他为矿物质和水。

應用

明胶(来源于猪皮)已用于:
  • 包被培养板玻璃底部,以便培养A75细胞和进行明胶降解分析
  • 包被玻片,以进行脑切片组织学研究
  • 合成甲基丙烯酸酯化明胶(GelMA)预聚物,用于细胞封装和3D打印水凝胶
  • 制备巯基化明胶(GelSH)水凝胶,用于3D生物打印(3D projection bioprinting)

明胶(来源于猪皮)建议以1-5 μg/cm2或0.5-50 μg/ml用作细胞培养基底。最佳的浓度的确取决于细胞类型以及应用和研究目标。

生化/生理作用

明胶聚合物可作为胶原的生物材料替代物,用于细胞和组织培养。作为精挑细选的胶凝剂,可用作增稠剂和食品配料。明胶还可用作发泡剂和黏合剂,以及用于医药行业。因生物可降解性和生物相容性,适合生物医药应用,尤其适合作为心血管组织再生的生物材料。

成分

明胶存在于胶原蛋白中,是一种具有高平均分子量的水溶性蛋白质的异质性混合物。 通过在水中将相关的皮肤、肌腱、韧带、骨骼等进行煮沸来提取蛋白质。 A类明胶来自于酸固化组织。 B类明胶来自于石灰固化组织。

注意

干燥的明胶,当在室温下储存于密闭容器中时,将可保持多年不变。 当在空气存在时在100℃下加热时,它会溶胀变软,并分解成碳质物质并伴有吡啶碱和氨的释放。

準備報告

该产品源自于猪皮。明胶可溶于热水而非冷水。 它几乎不溶于大多数有机溶剂,如醇、氯仿、二硫化碳、四氯化碳、乙醚、苯、丙酮和油。 由Bloom凝胶计所测定的Bloom指数,可表示由已知浓度溶液而形成的凝胶的强度。 Bloom指数与平均分子量成正比。 猪皮明胶的Bloom值在90至300 g之间。本品的胶冻强度达300,可溶于水(50 mg/mL)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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