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Merck

G1163

Sigma-Aldrich

O-糖苷酶 来源于肺炎链球菌

recombinant, expressed in E. coli, buffered aqueous solution

别名:

α-内切乙酰氨基半乳糖苷酶, O-糖酵素

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

共軛

(O-linked)

形狀

buffered aqueous solution

分子量

180 kDa

濃度

≥800 units/mL

運輸包裝

wet ice

儲存溫度

2-8°C

生化/生理作用

从糖蛋白上释放未取代的Ser-和Thr-连接β-Gal-(1→3)-α-GalNAc(核心1型O-聚糖)。 用唾液酸、乳糖胺(半乳糖-N-乙酰氨基葡萄糖)或岩藻糖取代的二糖核心将阻止水解并防止寡糖从蛋白质中释放出来。在使用O-糖苷酶切割之前,可能需要使用糖酵解酶进行预处理以从O-聚糖中去除取代糖。

包裝

提供5×反应缓冲液,250mM NaH2PO4,pH 5.0。

單位定義

在37 °C、pH 6.5下,一单位在每分钟内可水解1 μmole的硝基苯基半乳糖N-生物苷(β-Gal-(1→3)-α-GalNAc-1→ΟC6H4NO2)。

外觀

溶于50 mM磷酸钾,pH 7.5

分析報告

筛查是否存在:β-半乳糖苷酶、α-甘露糖苷酶、β-己糖胺酶、α-岩藻糖苷酶、神经氨酸酶和蛋白酶。有关特定批次的信息,请参阅分析证书。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析证书(COA)

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H Miyata et al.
Okajimas folia anatomica Japonica, 78(4), 129-140 (2002-01-05)
The distribution or localization of glycoconjugates in rat cerebellar cortex was investigated with 26 different kinds of lectins observed by light and electron microscopy. In paraffin-embedded tissues, PHA-L, PHA-E, DSA, WGA, ConA, LEA, LCA, PSA, and RCA-I, which mainly recognize
K M Hong et al.
Journal of cancer research and clinical oncology, 127(9), 551-558 (2001-09-26)
To identify a new tumor-associated antigen, a monoclonal antibody, SC142, was produced by immunizing mice with a stomach cancer cell line. The tumor specificity of mAb SC142 was studied by immunohistochemical staining, and the biochemical characteristics of this new gastrointestinal
L Robertson et al.
Journal of reproduction and fertility, 120(2), 397-403 (2000-11-04)
This study demonstrates that carbohydrates play an essential role in sperm-egg interactions in birds. Sperm-egg interaction was measured in vitro as the ability of spermatozoa to hydrolyse a small hole in the inner perivitelline layer, the equivalent of the mammalian
Aimee E Stephenson et al.
Molecular microbiology, 43(1), 147-157 (2002-02-19)
Streptococcus parasanguis is a primary colonizer of the tooth surface and plays a pivotal role in the formation of dental plaque. The fimbriae of S. parasanguis are important in mediating adhesion to saliva-coated hydroxylapatite (SHA), an in vitro tooth adhesion
C Liu et al.
Journal of mass spectrometry : JMS, 32(1), 33-42 (1997-01-01)
A high-performance liquid chromatographic/electrospray mass spectrometric (HPLC/MS) technique is described for the characterization of the beta-subunit of the glycopeptide human chorionic gonadotropin (hCG). The beta-subunit of hCG was dissociated from the alpha-subunit using 0.1% trifluoroacetic acid (TFA) and separated by

商品

While most N-linked oligosaccharides can be removed using PNGase F, a comparable enzyme for removing intact O-linked sugars has not been identified. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-β(1→3)-GalNAc core remains.

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